A sodium carbonate-bicarbonate buffer for alkaline phosphatases
نویسندگان
چکیده
منابع مشابه
A sodium carbonate-bicarbonate buffer for alkaline phosphatases.
For the study of alkaline phosphatase systems a non-phosphate-containing buffer is required. The buffers in common use for the alkaline pH range are ammonia, borate, glytine and veronal. Ammonia and borate have a retarding effect on the rate of ,enzymic hydrolysis of phosphoric esters. Glycine activates phosphatase when present in very low concentrations (0 1-1 mm), but in the concentrations us...
متن کاملThe biological buffer bicarbonate/CO2 potentiates H2O2-mediated inactivation of protein tyrosine phosphatases.
Hydrogen peroxide is a cell signaling agent that inactivates protein tyrosine phosphatases (PTPs) via oxidation of their catalytic cysteine residue. PTPs are inactivated rapidly during H(2)O(2)-mediated cellular signal transduction processes, but, paradoxically, hydrogen peroxide is a rather sluggish PTP inactivator in vitro. Here we present evidence that the biological buffer bicarbonate/CO(2)...
متن کاملP-80: The Effect of Alkaline Environments Made with Sodium BiCarbonate in order to Separate Spermatozoa for Sex Selection
Background: There are different factors Tahat effect in sex selection such as the best medium treatment.These techniques can be used in live stock industry for product in ordre to humans need. Materials and Methods: Forty mice (NMRI) were divided in to a control group and three experimental group. Ovulation of mice was stimulated by using PMSG and HCG hormones. Female mice were killed by cervic...
متن کاملCoral reef calcifiers buffer their response to ocean acidification using both bicarbonate and carbonate.
Central to evaluating the effects of ocean acidification (OA) on coral reefs is understanding how calcification is affected by the dissolution of CO(2) in sea water, which causes declines in carbonate ion concentration [CO(3)(2-)] and increases in bicarbonate ion concentration [HCO(3)(-)]. To address this topic, we manipulated [CO(3)(2-)] and [HCO(3)(-)] to test the effects on calcification of ...
متن کاملSodium bicarbonate
Major uses Sodium bicarbonate is a main constituent of baking soda/powder. Baking powder is composed of 30% of sodium bicarbonate, with cornstarch, sodium aluminium sulfate, calcium acid phosphate, and calcium sulfate [1]. Sodium bicarbonate may be an ingredient of folk remedies containing turpentine, castor oil, lemon and sugar. It is also added to mouthwashes and dentifrices to prevent dental...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1945
ISSN: 0306-3283
DOI: 10.1042/bj0390245